In the field of synthetic biology, artificial carbon assimilation pathways and artificial autotrophic systems are expected to contribute to the response to the environmental crisis and the promotion of sustainable material production. Achievements in this process, whether based on experience or model predictions, have contributed to the advancement of the field as a whole. As the cornerstone of biochemical reactions, enzymes have an important role in pathway implementation. At the same time, natural enzyme libraries offer a wide range of possibilities for mining pan-substrate activity or new functional mutants. Over the past decade, non-oxidative enzymes have stood out for their low-carbon profile and continue to show value in chassis development and product synthesis. Thus, summarizing and reviewing research advances in the field of artificial autotrophy can help readers identify potential research models and deepen their thinking when designing and selecting artificial pathways and their host systems. Essentially, the artificial carbon assimilation pathway corresponds to the dark reaction of photosynthesis, while its energy supply strategy corresponds to the light reaction. With the continuous adaptation of the two, artificial autotrophic systems will gradually evolve into innovative producers for industrial and environmental applications.
In a recent review in Biotechnology Advances the role of unnatural reactions and pathways in the development of artificial autotrophic systems were summarised. The Chinese reference contains appropriate graphs.