青岛 Qingdao
a hub for S&T

https://www.nature.com/articles/s41467-025-63929-7

http://english.cas.cn/newsroom/research_news/life/202510/t20251014_1089412.shtml

The group around Jian XU from the CAS Qingdao Institute of Bioenergy and Bioprocess Technology (QIBEBT) has developed a fully automated “Digital Colony Picker” (DCP). This device identifies and retrieves high-performance microbial clones by simultaneously monitoring their growth and metabolite production—eliminating the need for culture plates, sampling needles, or manual picking.

Designed for the “design-build-test-learn” framework widely adopted in synthetic biology, the DCP streamlines the traditionally slow, labor-intensive “test” phase into a fast, parallel workflow with little hands-on time. It has a microfluidic chip containing 16,000 addressable microchambers that isolate single cells and track their expansion into micro-colonies. An integrated AI engine conducts time-lapse analysis of both brightfield and biosensor signals to quantify growth kinetics and metabolite production in real time. Once target colonies are identified, a laser-induced bubble technique exports them as droplets directly into standard culture plates. This contact-free transfer minimizes cross-contamination and preserves cell viability.

The equipment which was tested for identifying high-yield or lactate-tolerant Zymomonas mobilis mutants is  broadly applicable to adaptive evolution studies, functional gene discovery, and phenotype screening across diverse microbial species.

https://doi.org/10.1016/j.ymben.2023.06.007

https://doi.org/10.1016/j.greenca.2023.08.001

https://www.guanhai.com.cn/p/39 4312.html

Trans-aconitic acid TAA (CAS RN 4023-65-8) is an unsaturated tricarboxylic acid that occurs in various plants. Although it exhibits broad application potential in agriculture, food, biomaterials, and green chemistry, its practical use remains limited. This is primarily because the traditional production processes of plant extraction (from sugar cane)and chemical synthesis (complex and inefficient) cannot achieve large-scale production at a low cost.

Researchers around LU Xuefeng, director of the Qingdao Institute of Bioenergy and Bioprocess Technology (QIBEBT) under the Chinese Academy of Sciences, have now established a cell factory for the production of TAA based on a genome-edited industrial strain of Aspergillus terreus. Several rounds of metabolic engineering resulted in strains which produced 57 g/L TAA in shake flask cultures. Scale-up to tank fermentations up to 120 kL – in cooperation with Shandong Lukang Pharmaceutical Co., Ltd.– then led to yields of 88 g/L after 100 hours. A simple recovery procedure combining membrane concentration and crystallization provided TAA crystals with a purity of 98.4%. Given its superior nematicidal properties, QIBEBT and Lukang Pharmaceutical are now in the process of registering TAA as a new nematicide biopesticide.

The QIBEBT team has further found that TAA esters (trans-Aconitates) can be used as plasticizers and could replace the ambiguous phthalates widely used in plastic products. Haier Blood Technology Co., a Qingdao-based company, plans to use TAA esters as plasticizers in its PVC-based blood bags and other products.

TAA ester’s wide temperature stability, from -46°C to 120°C, might also find applications in automotive cable materials as they exhibit excellent resistance to high-temperature volatilization and low-temperature brittle cracking.

In summary, biomanufacturing based on smart cells of A. terreus has provided a new material, TAA and TAA esters, which offer exciting application potentials as a biopesticide and a non-toxic bioplasticizer.

http://english.cas.cn/newsroom/research_news/life/202508/t20250801_1048868.shtml

https://biotechnologyforbiofuels.biomedcentral.com/articles/10.1186/s13068-025-02677-8

A team at CAS Qingdao Institute of Bioenergy and Bioprocess Technology (QIBEBT) has  developed a lipid-rich mutant strain of Saccharomyces cerevisiae for microbial production of palmitoleic acid— a rare omega-7 fatty acid with proven anti-inflammatory and metabolic benefits.

The team used a combined mutagenesis approach—employing zeocin, an antibiotic-based mutagen, and Atmospheric and Room Temperature Plasma (ARTP)—to create a diverse library of yeast mutants. They then deployed FlowRACS, a Raman flow cytometry system, to select live yeast cells with elevated lipid levels by analyzing their intrinsic single-cell Raman spectra, eliminating the need for chemical stains or genetic reporters.

This method identified the mutant strain MU2R48, which achieved a lipid content of 40.26%—a 30.85% increase over its parental strain SC018—while maintaining similar biomass production.

Photo: Raman flow cytometry efficiently identifies lipid-rich Saccharomyces cerevisiae  mutants from a Zeocin–ARTP-induced library. (Image by QIBEBT)