https://www.cas.cn/syky/202209/t20220927_4848957.shtml
https://www.pnas.org/doi/full/10.1073/pnas.2201473119
The team of Yongguan Zhu at the CAS Institute of Urban Environment has used single-cell Raman-D2O coupled with targeted metagenomics to develop a culture-independent approach to phenotypically and genotypically profiling active antibiotic-resistant bacteria against clinical antibiotics in a wide range of soils. This method quantifies the prevalence (contamination degree) and activity (spread potential) of soil antibiotic-resistant bacteria and reveals a clear elevation with increasing anthropogenic activities such as farming and the creation of pollution, thereby constituting a factor that is critical for the assessment of risks.
Single-cell Raman reveals soil-active antibiotic resistance groups of microorganisms