The team of LIU Shuangjiang took two years to construct the hGMB library. To this end, the research team picked more than 20,000 monoclonal clones from 239 fresh stool samples from healthy people of different regions and ages with physical examinations, and identified more than 10,000 pure culture isolates from them after expanded culture. Through macrogenomic analysis of the healthy human gut, 85 new species from this strain repository were found to occur in more than 90% of the healthy human gut, while covering 79% of the known common genera and 75% of the common species in the human gut. Last year, the team also published the largest model animal mouse gut microbial repository (mGMB) to date, containing 126 microorganisms and their genomes, of which 77 microorganisms are new species successfully isolated and cultured for the first time, increasing the culturable mouse gut microbial strain resources from 48 to 110 genera and from 76 to 180 species, and in the process, forming a set of In the process, a standardized procedure for the isolation and culture of anaerobic microorganisms and the description of species identification was developed.
