A novel artificial carbon fixation pathway LATCH with 10 enzymatic steps

https://www.sciencedirect.com/science/article/pii/S2950155525000667?via%3Dihub

https://www.cas.cn/syky/202511/t20251125_5089765.shtml

A research team at the CAS Tianjin Institute of Industrial Biotechnology has proposed a novel artificial carbon fixation pathway—LATCH which comprises 10 completely known enzymatic steps. Each cycle converts two molecules of HCO₃⁻ into one molecule of acetyl-CoA, requiring only adenosine triphosphate (ATP) and reduced coenzyme II for energy. Kinetic and thermodynamic modeling analysis shows that it is a linear autocatalytic cycle structure without kinetic traps or thermodynamic barriers, possessing high feasibility and potential for continued development. It can provide insights for improving the efficiency of systems such as photosynthetic microorganisms, plants, and engineered cell factories.

Regarding the selection of parental modules, the research team referenced research on the serine cycle and designed a modified version of the serine cycle, simplifying the pathway structure and bypassing the inefficient steps involving hydroxypyruvate, thus enabling the pathway to function effectively in the heterologous host *E. coli*. Simultaneously, the team replaced the amino acid deamination and transamination steps in the serine cycle with a decarboxylation process, forming an MCG cycle free from formic acid dependence. This cycle can further convert glycerate 3-phosphate produced by processes such as the Calvin cycle and glycolysis into acetyl-CoA in a negative carbon mode. The study also referenced a series of photorespiration bypass concepts developed for recovering the Rubisco byproduct glycolate-2-phosphate, among which the TaCo module, due to its artificial carboxylation reaction, theoretically has a maximum yield of 150%. This study found that by introducing glyoxylate reductase as a key step to act as a “molecular latch,” the natural serine cycle and the artificially carboxylated module TaCo can be recombined, resulting in a functional transformation—from two parent modules dependent on organic substrates to a complete carbon-fixing cycle.

Based on the LATCH cycle formed by module integration, kinetic analysis shows that this pathway is a linear autocatalytic cycle, theoretically avoiding kinetic traps while eliminating the need to establish complex regulatory relationships. Meanwhile, eight steps in the pathway receive thermodynamic support from adenosine triphosphate (ATP), reducing power, or high-energy substrates, and the remaining two lyase-catalyzed processes do not pose thermodynamic bottlenecks. These inherent advantages at the stoichiometric, kinetic, and thermodynamic levels lay the foundation for the continued development and application of LATCH.

more insights

An offshore platform for coastal ecosystem research near Qingdao

https://en.people.cn/n3/2026/0711/c90000-20476703.html

China put into operation a large offshore platform for controlled in-situ experiments regarding coastal ecosystems. Developed by the CAS Institute of Oceanology (IOCAS), the facility is deployed in the Yellow Sea off Rongcheng in Shandong Province.

With a total area of 2,000 square meters and an experimental sea zone of 30,000 square meters, the platform is designed to support a wide range of ecological studies under real marine conditions. Under real seawater conditions, key factors such as temperature, nutrients and dissolved oxygen can be precisely regulated, enabling continuous, full-cycle observation and breaking the technical barrier between natural-sea observation and indoor controlled experiments.

The platform comprises modules for automated monitoring, water regulation, mesocosm ecosystems and logistics support. It enables precise single-factor or multi-factor regulation of temperature, nutrients, dissolved oxygen and other variables, allowing researchers to reproduce scenarios like ocean warming, acidification and eutrophication in a real marine environment. This capability is crucial for understanding how coastal ecosystems respond to both gradual climate trends and extreme events.

China’s coastal waters, where most marine economic activities and ports are concentrated, are hotspots for ecological disasters and risks. These waters, notably, are under mounting stress from climate change, land-based inputs and human activities. The new lab is located in a typical mariculture area of northern China, offering conditions for both natural and farmed ecosystem studies. Researchers can conduct scenario experiments on carrying capacity, ecological resilience, marine heatwaves and hypoxia to identify risk thresholds and optimize aquaculture management. Beyond serving the aquaculture industry, it can provide technical support for disaster prevention, precision restoration and coastal health management, said Sun.

The platform is designed for open international collaboration and will be made available to global research institutions, facilitating joint efforts to tackle pressing marine environmental challenges.

Photo: An aerial drone photo taken on July 10, 2026 shows the large offshore platform for controlled in-situ experiments on coastal ecosystems after being deployed in the Yellow Sea off Rongcheng in east China’s Shandong Province. China on Friday put into operation a large offshore platform for controlled in-situ experiments regarding coastal ecosystems, marking a new phase of systematic, intelligent and open coastal ecological research in the country. Developed by the Institute of Oceanology under the Chinese Academy of Sciences (IOCAS), the facility is deployed in the Yellow Sea off Rongcheng in east China’s Shandong Province. It is the country’s largest open-access facility for in-situ coastal experiments, the IOCAS said. (IOCAS/Handout via Xinhua)

Green Carbon journal reaches impact factor 14.2 in less than 3 years

https://www.sciencedirect.com/journal/green-carbon

Green Carbon has received its first Impact Factor of 14.2 in the 2025 Journal Citation Reports (JCR) released by Clarivate on June 17, 2026. This places Green Carbon in Q1 in both the “Engineering, Chemical” category (Ranked 10/183) and the “Green & Sustainable Science & Technology” category (Ranked 8/114). Achieving this in less than three years since its launch is a testament to the journal’s academic quality, rigorous publishing standards, and growing international influence.

A deep-sea isopod uses horizontal gene transfer with a symbiotic bacterium to withstand ultra-long starvation

https://english.news.cn/20260606/de8eff009a94407c8eeeb1fdab13d675/c.html

https://www.cell.com/cell/abstract/S0092-8674(26)00571-4?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS0092867426005714%3Fshowall%3Dtrue

A joint research led by the CAS Institute of Oceanology in collaboration with the Hong Kong-based Chinese University of Hong Kong and Northwestern Polytechnical University in Xi’an deciphered the mechanism of ultra-long starvation tolerance in deep-sea isopods and provides an important paradigm for understanding how life balances growth and survival in extreme environments.

The deep sea is cold, dark, and almost entirely devoid of reliable nutrition, making long-term survival a remarkable evolutionary feat. To survive the abyss, the isopod possesses an enormous stomach that occupies about two-thirds of its body and acts like a deep-freeze pantry, allowing it to gorge when food is available and store the haul for months or even years. Second, it maintains an exceptionally low basal metabolic rate, essentially putting itself on permanent energy-saving mode. Together, these traits turn opportunistic binge eating into an ultra-long energy reserve.

In addition, a key gene involved in this metabolic slowdown, named ND1, is not originally part of the isopod’s own genome. The isopod “hijacks” it from an external symbiotic bacterium through horizontal gene transfer.

To verify ND1’s function, the researchers inserted the gene into zebrafish, nematodes, and human cells in the lab. Under normal temperatures, the gene recipients burned energy faster and became less tolerant of starvation. However, under cold conditions that mimic the isopod’s deep-sea home, ND1 suppressed energy metabolism, reduced mitochondrial activity, and boosted starvation endurance in zebrafish by a remarkable 37 percent.

This temperature-dependent switch solves the so-called “energy paradox” — how can a giant animal with high energy demands survive where food is extremely scarce? The ND1 acts as a metabolic thermostat, fine-tuning energy burn in response to environmental conditions. It provides a solution to the trade-off between body size and food scarcity.

Back to …

青岛 Qingdao
a hub for S&T