https://www.cas.cn/syky/202509/t20250905_5081397.shtml

The CAS Center for Excellence in Molecular Cell Science has developed a high-throughput single-cell genome and transcriptome sequencing method, wellDR-seq, which ​​performed dual-omics analysis on over 30,000 single cells from 12 breast cancer cases. Using this method, they were able to identify and characterize the tumor-initiating ancestral subclones of ER-positive breast cancer, quantitatively describing the complex dosage-regulatory relationship between gene copy number changes and gene expression changes at the single-cell level. This study provides new theoretical basis and technical approaches for early diagnosis, prognosis, and precision treatment of breast cancer.

The research team developed wellDR-seq, a high-throughput, high-resolution single-cell DNA and RNA co-sequencing method. This method, which repeatedly optimizes the biochemical reactions of DNA and RNA and combines dual-index labeling with nanopore microarray technology, enables the first high-precision co-sequencing of thousands of single-cell genomes and transcriptomes simultaneously.A