https://www.cell.com/plant-communications/fulltext/S2590-3462(24)00042-7
https://www.cas.cn/syky/202402/t20240207_5005253.shtml
A team at South China Botanical Gardenias developed RAPID, a fast and efficient plant genetic transformation methods, based on active regeneration capacity of plants. They found that positive organs and transformed individuals could be obtained rapidly by injection into the sweet potato stem segment, and transformation efficiency could be optimized in terms of Agrobacterium strains, infiltration concentration, chemoactivators and other methodological conditions, They developed a variety of reporter genes and genetic editing tools, and then confirmed the plant meristematic tissues by the analysis method of genetics and cytology Efficient transfection as well as rapid regeneration of individual newborn transformed organs. The RAPID method has been applied to asexually reproduced cash crops or resource plants such as sweet potato, potato and thick vine.
Compared with traditional genetic transformation methods, the RAPID method has higher transformation efficiency (20-100 times higher), simpler operation process (2-3 times shorter), and does not require expensive and complicated tissue culture process.The RAPID method overcomes the limitations of the traditional method in rapid genetic transformation, and breaks the bottleneck in the development and utilization of various types of resource plants. Based on the active regeneration ability and asexual reproduction, this method is expected to be applied to a wider range of plant species, and to realize the trait improvement and germplasm innovation of more characteristic resource plants and economic crops, so as to promote the development of basic and applied research in related fields, and to provide theoretical and methodological support for the efficient genetic improvement of characteristic plants and the application of modern agricultural breeding.