The group of WANG Ming has developed a new CRISPR/Cas9 genome editing procedure based on the molecular self-assembly between cationic liposomes and Cas9 messenger RNA (Cas9 mRNA) nanoparticles. They showed that after the mRNA nanoparticles are injected through the tail vein of a mouse and are in mouse parenchymal cells, they are degraded by reduced glutathione in the cell, releasing Cas9 mRNA and translating Cas9 nuclease for gene editing. Using this method, GFP gene editing efficiency was as high as 90%).Cas9 mRNA nanoparticles.

CAS news release, December 27, 2019