https://www.cas.cn/syky/202601/t20260121_5096791.shtml

https://www.sciencedirect.com/science/article/abs/pii/S1096717626000042?via%3Dihub

A research team at the CAS Tianjin Institute of Industrial Biotechnology has established  an in vitro metabolic engineering technique that achieves efficient, coenzyme-free synthesis of β-1,2-glucan from cellobiose. T

Through the synergistic action of cellobiose phosphorylase, β-1,2-oligoglucan phosphorylase, glucose oxidase, and catalase, glycosidic bond rearrangement was achieved without the need for coenzymes such as CoA and ATP, or external energy input. The molar yield reached 93.3%, and the production rate reached 4.0 g/L/h. A highly active, thermostable β-1,2-oligoglucan phosphorylase from thermophilic bacteria was identified and characterized, exhibiting superior catalytic efficiency and thermostability compared to previously reported enzymes. A pH self-neutralization regulation system was established. By optimizing parameters such as primer concentration and enzyme ratio, precise control of the degree of polymerization was achieved, resulting in a product dispersibility index as low as 1.11, surpassing current industry standards.

β-1,2-glucans have broad applications in biocatalysis, drug delivery, and food additives.Coenzym