A combination of FISH and Raman sequencing allows for enzyme detection in environmental samples

http://english.cas.cn/newsroom/research_news/life/202501/t20250110_898273.shtml

https://doi.org/10.1016/j.xinn.2024.100759

A new technology termed FISH-scRACS-seq (Fluorescence In Situ Hybridization-guided Single-Cell Raman-activated Sorting and Sequencing) combines species-targeting fluorescence in situ hybridization (FISH) with Raman spectroscopy, allowing for the direct identification and isolation—from environmental samples—of functional single cells and the enzymes they encode.

The research team utilized this technique to identify the cells, pathways, and enzymes from γ-proteobacteria that are actively involved in degrading cycloalkanes in marine environments. Their analysis uncovered a previously unknown P450 enzyme encoded by Pseudoalteromonas fuliginea, crucial for bioremediation efforts in aquatic ecosystems contaminated by hydrocarbons.

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A humanoid robot training center in Qingdao improves robot efficiency

https://en.people.cn/n3/2026/0324/c90000-20439477.html

At the Qingdao Humanoid Robot Data Training Center in Laoshan District of Qingdao, humanoid robots are trained for jobs such as intelligent industrial manufacturing, smart home, and commercial services. Data collectors here control robots to complete specific tasks like logistics sorting, supermarket restocking, kitchen operations, and component assembly. Through thousands of repetitions and trials, massive amounts of motion data are generated, endowing robots with a smarter “intelligent brain,” and helping humanoid robots enter all walks of life to serve thousands of households.

RamEx: high-throughput microbial ramanome analyses with accurate quality assessment

https://www.cas.cn/syky/202602/t20260226_5102870.shtml

https://doi.org/10.1186/s40168-026-02339-3

A research team at the CAS Qingdao Institute of Bioenergy and Bioprocess Technology has developed RamEx, an integrated analysis framework for Ramanome big data. This platform, tailored to the characteristics of Raman spectroscopy data, establishes a one-stop workflow from data reading and standardized preprocessing to downstream data mining, centered on automated quality control algorithms and efficient parallel computing processes. It also demonstrates a systematic analysis of microbial metabolomical heterogeneity and metabolic pattern differentiation at the single-cell level.

Raman genomics deep analysis can track the dynamic changes in the composition of macromolecules such as lipids, proteins, and nucleic acids in different cells, thus revealing the differentiation and succession patterns of microbial metabolic states at the population scale with single-cell precision. This provides new research ideas and technical pathways for understanding the functional organization and environmental adaptation mechanisms of complex communities.

RamEx removes spectral noise in Raman-based microbial cell sorting

http://english.cas.cn/newsroom/research-news/202602/t20260224_1151116.shtml

https://link.springer.com/article/10.1186/s40168-026-02339-3

Scientists from the CAS Qingdao Institute of Bioenergy and Bioprocess Technology have developed a novel computational tool, RamEx, designed to resolve the computational bottleneck in high-throughput microbial Ramanomics.

RamEx streamlines the full Ramanomic analysis pipeline, from data preprocessing and automated quality control to advanced data mining. An Iterative Convolutional Outlier Detection (ICOD) algorithm tackles spectral noise in an unsupervised manner to dynamically identify and eliminate spectral artifacts, ensuring high-quality input for downstream analysis.

The platform’s performance was validated using diverse datasets, including pathogenic bacteria, probiotics, and yeast fermentation systems. Notably, RamEx successfully captured phenotypic heterogeneity in genetically identical yeast cells by detecting subtle metabolic fluctuations and tracking the dynamic accumulation of intracellular macromolecules, including lipids, proteins, and nucleic acids.

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