A group around Weihong JIANG at CAS Institute of Plant Sciences in Shanghai metabolically engineered autotrophic Clostridium ljungdahlii to efficiently co-produce three important bulk chemicals, isopropanol, 3-hydroxybutyrate (3-HB), and ethanol (together, IHE), using syngas (CO2/CO). The engineered strain was further optimized to reassimilate acetic acid and synthesize 3-HB by introducing heterologous functional genes. Finally, the best-performing strain was able to produce 13.4, 3.0, and 28.4 g/L of isopropanol, 3-HB, and ethanol, respectively, in continuous gas fermentation.

https://doi.org/10.1021/acssynbio.1c00235

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