A group around Weihong JIANG at CAS Institute of Plant Sciences in Shanghai metabolically engineered autotrophic Clostridium ljungdahlii to efficiently co-produce three important bulk chemicals, isopropanol, 3-hydroxybutyrate (3-HB), and ethanol (together, IHE), using syngas (CO2/CO). The engineered strain was further optimized to reassimilate acetic acid and synthesize 3-HB by introducing heterologous functional genes. Finally, the best-performing strain was able to produce 13.4, 3.0, and 28.4 g/L of isopropanol, 3-HB, and ethanol, respectively, in continuous gas fermentation.
https://doi.org/10.1021/acssynbio.1c00235
27-9 CAS Shanghai converts syngas aerosols to medium- and long-chain chemicals using Clostridium ljungdahlii