The team around XU Jian at the Single Cell Research Center of CAS Qingdao Institute of BioEnergy and Environmental Technology (QIBEBT) has combining tumor cell Raman group collection and multivariate curve resolution-alternating least square analysis algorithms to elucidate drug sensitivity detection on tumor single cells. Using rapamycin as an antitumor agent which interferes with mTOR signaling, they observed trhat in the lipid bodies of cancer cells, metabolic activities of both protein and lipid were suppressed; in the nucleus, protein synthesis remained active, whereas lipid synthesis was inhibited; and in the cytoplasm, syntheses of protein and lipid were both dose- and duration-dependent. Thus, rapamycin differentially influences protein and lipid synthesis in mTOR signaling.
CAS news release, January 18, 2021, and https://doi.org/10.1021/acs.analchem.0c03925
