The group of ZHU Yuming has developed a one-pot two-enzyme system. It is based on a new type of exo-inulinase derived from Bacillus velezensis, which achieves efficient hydrolysis of inulin with a hydrolysis efficiency of 98 %, followed by epimerization with D-allulose 3-epimerase from Ruminococcus sp., which was coexpressed with the exo-inulinase as a recombinant extracellular enzymes in B. subtilis. Based on the two inexpensive enzymes, the ratio of D-glucose, D-fructose, and D-allulose in the product was approximately 1:3:1, and the yield on JA powder was 67%.
https://doi.org/10.1016/j.procbio.2019.10.006
CAS Institute of Industrial Biotechnology develops cheap process for production of D-psicose sweetener