CAS Tianjin Institute of Industrial Biotechnology reports progress in Aspergillus niger genome editing

The group around SUN Jibin used a ribosomal 5S rRNA gene as a promoter to mediate the expression of sgRNA in a novel and efficient CRISPR/Cas9 system which enabled Cas9 to cut 100% of the Aspergillus niger genome. This allowed to establish an efficient Aspergillus niger genome editing toolkit. With a 40 bp short homology donor DNA, single- and multi-site knock-ins and large 48-kb DNA knockout genomes obtained with accurate editing. The new CRISPR/Cas9 approach effectively solved the problem that Aspergillus niger espresses sgRNA activity.

CAS news release, May 3, 2018

CAS Tianjin Institute of Industrial Biotechnology reports progress in Aspergillus niger genome editing
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