9-7 CAS Dalian Institute of Chemical Physics establishes metabolic engineering platform of Pichia pastoris
ZHOU Yongjin and colleagues developed novel genetic tools for precise genome editing in Pichia pastoris by enhancing homologous recombination (HR) rates and engineering the multiple intrusion-induced rearrangement (MIR) processes. The key gene RAD52, which played crucial role in HR repair in Pichia pastoris, was overexpressed for improving the efficiency of single gene editing to 90%. […]
